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Rezeda I. Nazarova¹, Elmira M. Kosacheva², Roza F. Bakeeva¹*, Sergey Yu. Garmonov¹

Author affiliations
¹Department of Analytical Chemistry, Kazan National Research Technological University, Kazan, Russia
²Kazan Cooperative Institute (filial) Russian University of Cooperation, Kazan, Russia
*Corresponding author: gurf71@mail.ru

Abstract
Results of a comparative analysis of micellar and polymer colloidal matrices for the analysis of nitrofural (NF) content by spectrophotometric method in pharmaceutical substances and dosage forms (DF) using the method of response surface (MRS) and three-level Box-Behnken plans are presented. The system N-decylpyridinium chloride (NDPC) + dimethyl sulfoxide (DMSO) + H₂O (I) was used as a micellar matrix, and polyethyleneimine (PEI) + NDPC + DMSO + H₂O (II) was used as the polymer colloid matrix. The target function in both cases was the intensity of the absorption band at 375 nm, A₃₇₅, reflecting NF content. Independent factors were surfactant concentration (C_NDPC, mol/L), pH of medium, and fraction of DMSO (α_DMSO). In the matrix (I), optimal conditions for finding the NF content are С_NDPC = 0.032 mol/L; pH = 2; αDMSO = 0.094. In the presence of PEI, the amounts of NDPC and DMSO are significantly less (С_NDPC = 0.001 mol/L; pH = 7.6; α_DMSO = 0.014, С_PEI = 0.02 mol/L). In both systems, a molar extinction coefficient is ˜ 17,000 L·cm^-1·mol^-1, which allow to obtain reliable analytical characteristics. The solubilization of NF in the systems I and II was considered. The fundamental possibility of using a system based on PEI for microencapsulation of NF and in delivery vehicles are demonstrated.

Keywords: nitrofural, N-decylpyridinium chloride, polyethyleneimine, dimethyl sulfoxide, micelles, polymer colloidal complexes, analytical characteristics, response surface methodology, Box-Behnken plans