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Zhidk. krist. ikh prakt. ispol'z. = Liq. Cryst. and their Appl., 2014, 14 (4), 5—21.
Gold Nanoparticles Influence Double-Stranded DNA Molecules "Recognition" and Prevent Formation of Their Cholesteric Structure
Yu. M. Yevdokimov1, S. G. Skuridin1, V. I. Salyanov1, V. I. Popenko1,
E. V. Shtykova2, N. G. Khlebtsov3, G. A. Shafeev4, E. I. Kats5
Author affiliations 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences,
Vavilov str., 32, 119991 Moscow, Russia. E-mail: firstname.lastname@example.org 2Shubnikov Institute of Crystallography, Russian Academy of Sciences,
Leninsky ave., 59, 119333 Moscow, Russia. E-mail: email@example.com 3Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Entuziastov ave., 13, 410049 Saratov, Russia. E-mail: firstname.lastname@example.org 4Prokhorov General Physics Institute, Russian Academy of Sciences,
Vavilov str., 38, 119991 Moscow, Russia. E-mail: email@example.com 5Landau Institute for Theoretical Physics, Russian Academy of Sciences,
Kosygin str., 2, 119334, Moscow, Russia. E-mail: firstname.lastname@example.org
Abstract The negatively charged gold nanoparticles (nano-Au) in solutions of high ionic strength interact with free, linear double-stranded (ds) DNA or synthetic poly(ribonucleotide) (poly(I)×poly(C) or poly(A)×poly(U)) molecules or with these molecules ordered in the spatial structure of the cholesteric liquid-crystalline dispersion (CLCD) particles formed as a result of their phase exclusion from solutions containing poly(ethylene glycol). However, the consequences of these interactions differ noticeably. If we first pretreate free ds nucleic acid (NA) molecules with nano-Au and then create dispersion, the orientationally ordered structures do not form. This leads to disappearance of abnormal band in the circular dichroism spectra at a very low concentration of added nano-Au. If we treat condensed ds NA, i.e. ds NA in the content of CLCD particles, with nano-Au, the orientational order remains stable. This order evolves (from cholesteric to untwisted, nematic-like) upon increase in nano-Au concentration. The preliminary measurements and evaluations show that the analogous spectral changes are also typical for palladium nanoparticles. The differences in nano-Au action on free and condensed ds NA molecules can result in various damaging effects in living cells and be accompanied by various genetic consequences.
Keywords: nanoparticles of noble metals; free double-stranded nucleic acids; circular dichroism of nucleic acid molecules; cholesteric liquid-crystalline DNA dispersions; cholesteric → nematic transition; aggregated DNA molecules